Part:BBa_K3598009
tridecapeptide
A short antimicrobial peptide containing 13 amino acids.
Experiments and Results
The tridecapeptide coding part is the original sequence of tridecapeptide, which is a quite short antimicrobial pepetide. We verified its antimicrobial potency through two methods: plate inhibition zones assay and OD600 analysis. In plate inhibition zones assay,wo placed pieces of paper soaked with AMP solutions onto plates inoculated with E.coli MG1655 and P.acnes and observing bacteria growth inhibition zones. In OD600 analysis, we added AMP solutions into liquid culture inoculated with the bacteria and measuring OD600 value.
Plate verification Results
The plate results showed inhibition zones in areas of direct contact between bacteria and tridecapeptide, which means tridecapeptide is effective in killing both E.coli and P.acnes, while would not affect bacteria outside its contact. However, the edge of the zone of inhibition is not clear when test to E.coli MG655，which means tridecapeptide’s efficiency to E. coli is not significant. Additionally, the zone of inhibition in P.acnes plate was blurry, that may due to the hydrophobicity of this short peptide.
OD600 Verification
Then, we verified tridecapeptide's ability to kill E. coli MG1655 and P. acnes by adding its solution to liquid culture inoculated with the two bacteria. We set the AMP concentration gradient as 0, 2, 4, 8, 12, 16mg/L, and measured the OD600 of the E. coli through time. Since P. acnes has a slow growth rate, we only measured OD600 after 48 hours of cultivation.
The results (Figure 5 & 6）indicate that tridecapeptide's efficiency to E. coli MG1655 were not significant, only showed obverse peak at 8 h. Its ability to kill P. acnes was relatively obvious at concentration 16mg/L, the final OD600 was about 14.6% decreased.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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